Viability and discrimination of avian peripheral blood mononuclear cells and thrombocytes intended for improvement of wound healing in birds

Abstract

Birds often suffer from skin injuries of different aetiology. Platelet-rich plasma (PRP) is successfully used for improvement of wound healing in humans and in some mammalian species (e.g. horses, dogs and cats), but experience with its application in avian patients has not yet been published. Therefore, the aim of this study was to test a quick method for the counting of isolated avian platelets and mononuclear leukocytes and to find an appropriate carrier for their application to the wounds of birds. It seems that flow cytometry can be used for the quick counting of isolated cells and the discrimination of thrombocytes, lymphocytes and eventually monocytes or debris. Of the tested gels and sponges routinely used for improvement of wound healing, a gelatin sponge (Gelaspon®) providing the highest numbers and viability of isolated cells proved to be the best carrier.