Affiliation:
1. 1 Institute of Veterinary Medicine of Serbia, Vojvode Department for Swine Diseases Toze 14 11 000 Belgrade Serbia
2. 2 University of Novi Sad Faculty of Agriculture, Department for Veterinary Medicine Novi Sad Serbia
Abstract
Mycoplasma hyopneumoniae
is a primary agent associated with mycoplasma pneumonia and the porcine respiratory disease complex (PRDC). Various reports have indicated that different strains of
M. hyopneumoniae
are circulating in the swine population. Lysates from lung swabs from naturally infected pigs of different ages were tested according to a new variable number of tandem repeats (VNTR) genetic typing method based on the polyserine repeat motif of the P146 lipoproteoadhesin, which can be applied directly on clinical material without isolation of
M. hyopneumoniae
. The aim was to determine the diversity of
M. hyopneumoniae
isolates from conventional farrow-to-finish pig farms located in different geographical areas of Serbia. PCR amplification was carried out using
M. hyopneumoniae
-specific designed, conserved primers (p146MH — L and p146MH — R) flanking the region encoding the repeat motif, followed by sequencing and cluster analysis. Five groups of
M. hyopneumoniae
with thirteen to twenty-four serine repeats were observed. Analysis of three samples from each farm indicated that the specific isolate is ubiquitous in pigs of different ages. Furthermore, seven clusters were observed within 27 tested samples. The results indicated a considerable diversity among
M. hyopneumoniae
field isolates in the swine population from conventional farrow-to-finish farms in Serbia and suggest close genetic relatedness of the corresponding isolates.
Reference31 articles.
1. Arbitrarily primed PCR analysis of Mycoplasma hyopneumoniae field isolates demonstrates genetic heterogeneity;Artiushin S.;Int. J. Syst. Bacteriol.,1996
2. Emergency-culling and mortality in growing/fattening pigs in a large Hungarian ‘farrow-to-finish’ production unit;Baumann B.;Dtsch. Tierärztl. Wschr.,2002
3. Porcine Respiratory Disease Complex (PRDC): A review. I. Etiology, epidemiology, clinical forms and pathoanatomical features;Bochev I.;Bulg. J. Vet. Med.,2007
4. Protein variability among Mycoplasma hyopneumoniae isolates;Calus D.;Vet. Microbiol.,2007
5. Assessing Mycoplasma hyopneumoniae aerosol movement at several distances;Cardona A. C.;Vet. Rec.,2005
Cited by
8 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献