Affiliation:
1. 1 Tehran University of Medical Sciences Department of Microbiology, School of Medicine Tehran Iran
2. 2 Tehran University of Medical Sciences Maternal, Fetal and Neonatal Research Center, Vali-asr Hospital Tehran Iran
3. 3 Pars Hospital Department of Microbiology Tehran Iran
4. 4 Ilam University of Medical Sciences Clinical Microbiology Research Center Ilam Iran
Abstract
Forty-one Streptococcus agalactiae isolates collected from pregnant women at 35–37 weeks of gestation were analysed for their capsular types, antimicrobial resistance determinants, distribution of virulence factors and genetic relatedness using PCR and multiplex PCR. Capsular type III was predominant (65.8%), followed by capsular type II (14.6%), Ib (7.3%), and V(4.9%). All isolates were susceptible to penicillin, vancomycin, linezolid and quinupristin-dalfopristin. Resistance to tetracycline, erythromycin and clindamycin were found in 97.6%, 24.4%, and 14.6% of isolates, respectively. The most common antimicrobial resistance gene was tetM found in 97.6% of the isolates followed by ermTR and ermB found in 12% and 7.3% of isolates, respectively. The most common virulence gene was hly (100%), followed by scpB (97.6%), bca (97.6%), rib (53.65%) and bac (4.9%). The insertion sequence IS1548 was found in 63.4% of isolates. By multi locus variable number of tandem repeat analysis (MLVA) typing, 30 different allelic profiles or MLVA types (MTs) were identified. The most frequent was the MT1 (5/41, 12.2%) and followed by MT2 (4/41, 9.75%). Our data revealed that population structure of these isolates is highly diverse and indicates different MLVA types.
Subject
General Immunology and Microbiology,General Medicine
Reference38 articles.
1. High incidence of erythromycin resistance among clinical isolates of Streptococcus agalactiae in Taiwan;Hsueh P.R.;Antimicrob Agents Chemother,2001
2. Surface proteins of Streptococcus agalactiae and related proteins in other bacterial pathogens;Lindahl G.;Clin Microbiol Rev,2005
3. Molecular epidemiology of group B streptococci in Ireland: Associations between serotype, invasive status and presence of genes encoding putative virulence factors;Dore N.;Epidemiol Infect,2003
4. Commensal Streptococcus agalactiae isolated from patients seen at University Hospital of Londrina, Parana, Brazil: Capsular types, genotyping, antimicrobial susceptibility and virulence determinants;Otaguiri E.S.;BMC Microbiol,2013
5. Prevention of perinatal group B streptococcal disease revised guidelines from CDC, 2010;Verani J.R.;MMWR Recomm Rep,2010
Cited by
29 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献