Flax-seed oil and Lactobacillus plantarum supplementation modulate TLR and NF-κB gene expression in enterotoxigenic Escherichia coli challenged gnotobiotic pigs

Author:

Chytilová Mária1,Nemcová Radomíra1,Gancarčíková Soňa1,Mudroňová Dagmar1,Tkáčiková Ľudmila1

Affiliation:

1. 1 University of Veterinary Medicine and Pharmacy Department of Microbiology and Immunology Komenského 73 041 81 Košice Slovak Republic

Abstract

The present study analyses the effect of flax-seed oil rich in n-3 polyunsaturated fatty acids (PUFAs), the probiotic strain Lactobacillus plantarum — Biocenol™ LP96 and their combination on the expression level of selected Toll-like receptor (TLR) genes (TLR2, TLR4, TLR5, TLR9) and their downstream molecules (myeloid differentiation factor 88, MyD88; nuclear factor-κB, NF-κB) in the jejunum of gnotobiotic pigs challenged with enterotoxigenic Escherichia coli (ETEC). The results show that both immunomodulators are able to modulate the RNA level of at least one of the target molecules and thus regulate pathogeninduced inflammation. We confirmed that not only probiotic lactobacilli or flaxseed oil alone but also their synergistic action has great potential in the prevention and treatment of porcine colibacillosis. The results give an insight into one of the possible mechanisms by which natural agents, such as probiotic lactobacilli and flax-seed oil, exert their immunoregulatory properties during pathogen-induced inflammation.

Publisher

Akademiai Kiado Zrt.

Subject

General Veterinary

Reference27 articles.

1. Pathogen recognition and innate immunity;Akira S.;Cell,2006

2. The influence of omega-3 polyunsaturated fatty acids (omega-3 PUFA) on lactobacilli adhesion to the intestinal mucosa and on immunity in gnotobiotic piglets;Bomba A.;Berl. Münch. Tierärztl. Wschr.,2003

3. TLR2 activation is essential to induce a Th1 shift in human peripheral blood mononuclear cells by plant stanols and plant sterols;Brüll F.;J. Biol. Chem.,2010

4. Anti-inflammatory and immunoregulatory effects of flax-seed oil and Lactobacillus plantarum — Biocenol™ LP96 in gnotobiotic pigs challenged with enterotoxigenic Escherichia coli;Chytilová M.;Res. Vet. Sci.,2013

5. Strong immunostimulation in murine immune cells by Lactobacillus rhamnosus GG DNA containing novel oligodeoxynucleotide pattern;Iliev I. D.;Cell. Microbiol.,2005

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3