Aflatoxin M1 binding by probiotic bacterial cells and cell fractions

Abstract

AbstractFood industrial bacterial cells eliminate aflatoxin M1 (AFM1) at different ratios. The study aimed to investigate the effect of AFM1 on probiotic industrial bacteria (Lactococcus lactis ssp. lactis R703, Bifidobacterium animalis ssp. lactis BB12, and L. paracasei subsp. paracasei 431) and evaluating their AFM1 binding ability in naturally contaminated milk. The growth of the R703 strain was affected by AFM1 at 1.47 μg L−1 concentration. Peptidoglycan (PG) cell wall fractions of R703 and BB12 bound a significant amount of AFM1 from naturally contaminated milk under one-hour treatment, while L. paracasei 431 was not effective. PG was better absorbent for AFM1 than viable cells of BB12, while the difference was insignificant for the R703 strain. Increasing the time did not significantly change the mycotoxin binding of BB12, while for R703 PG the absorption seemed reversible. BB12 PG needs further analysis for biotechnological application in dairy products.