Binding of mycoplasmas to solid phase adsorbents-Reference-Cited by-同舟云学术

Binding of mycoplasmas to solid phase adsorbents

Published:2005-08-01 Issue:3 Volume:53 Page:299-307
ISSN:0236-6290
Container-title:Acta Veterinaria Hungarica
language:
Short-container-title:

Author:

Szathmáry Susan¹,Rajapakse Nandani²,Székely Ibolya³,Pitlik E.⁴,Bíró Judit⁵,Erdei Noémi⁶,Stipkovits L.⁷

Affiliation:

1. 1 Clarigen Inc. Carlsbad, CA, USA

2. 2 Clarigen Inc. Carlsbad, CA, USA

3. 3 Veterinary Medical Research Institute of the Hungarian Academy of Sciences H-1581 Budapest, P.O. Box 18, Hungary

4. 4 Second Department of Internal Medicine, Semmelweis University Budapest, Hungary

5. 5 Veterinary Medical Research Institute of the Hungarian Academy of Sciences H-1581 Budapest, P.O. Box 18, Hungary

6. 6 Veterinary Medical Research Institute of the Hungarian Academy of Sciences H-1581 Budapest, P.O. Box 18, Hungary

7. 7 Veterinary Medical Research Institute of the Hungarian Academy of Sciences H-1581 Budapest, P.O. Box 18, Hungary

Abstract

The capture of mycoplasmas(M. hominis, M. buccale, M. fermentans, M. bovis, M. synoviae, M. gallisepticumandM. arthritidis)based on lipid structures and adhesion molecules present in the mycoplasmal membrane was tested using different chromatographic resins (ActiClean Etox, ClarEtox, Heparin-Actigel, Sulfated Hiflow and SulfEtox). All of the resins efficiently reduced mycoplasma concentrations in Phosphate Buffered Saline (PBS) and in Fetal Bovine Serum (FBS) by 3-8 logs in a few minutes. This technology could be used for removing mycoplasmas from tissue culture components such as serum, and for concentrating mycoplasmas in vaccine production.

Publisher

Akademiai Kiado Zrt.

Subject

General Veterinary

Link

https://www.akademiai.com/doi/pdf/10.1556/avet.53.2005.3.3

Reference30 articles.

1. Papillomavirus microbicidal activities of high-molecular weight cellulose sulfate, dextrane-sulfate and polystyrene sulfonate.;N. Christensen;Antimicrob. Agent Chemother.,2001

2. Protection against airsacculitis with sequential systemic and local immunization of chickens using killed Mycoplasma gallisepticum bacteria with iota carrageenan adjuvant.;M. Elfaki;Vaccine,1993

3. Bovine mycoplasmas: Culture and biochemical studies.;H. Ernø;Acta Vet. Scand.,1973

4. Adherence of Mycoplasma pneumoniae to human alveolar macrophages.;A. Athamna;FEMS Immunol. Med. Microbiol.,1996

Cited by 1 articles. 订阅此论文施引文献订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献

1. Mycoplasma Mastitis;Veterinary Clinics of North America: Food Animal Practice;2012-07