Simultaneous quantitation of nicotinamide riboside and nicotinamide in dietary supplements via HPTLC–UV with confirmation by online HPTLC–ESI

Simultaneous quantitation of nicotinamide riboside and nicotinamide in dietary supplements via HPTLC–UV with confirmation by online HPTLC–ESI–MS

Published:2020-06 Issue:2 Volume:32 Page:128-133
ISSN:2083-5736
Container-title:Acta Chromatographica
language:
Short-container-title:Acta Chromatographica

Author:

Neamţu Andreea Silvia¹,Biţă Andrei²,Scorei Ion Romulus³,Rău Gabriela⁴,Bejenaru Ludovic Everard²,Bejenaru Cornelia⁵,Rogoveanu Otilia-Constantina⁶,Oancea Carmen Nicoleta¹,Radu Antonia⁵,Pisoschi Cătălina Gabriela⁷,Neamţu Johny⁸,Mogoşanu George Dan²

Affiliation:

1. 1 Doctoral School, University of Medicine and Pharmacy of Craiova, 2 Petru Rareş Street, 200349 Craiova, Dolj County, Romania

2. 2 Department of Pharmacognosy & Phytotherapy, Faculty of Pharmacy, University of Medicine and Pharmacy of Craiova, 2 Petru Rareş Street, 200349 Craiova, Dolj County, Romania

3. 3 BioBoron Research Institute, S.C. Natural Research S.R.L., 31B Dunării Street, 207465 Podari Commune, Dolj County, Romania

4. 4 Department of Organic Chemistry, Faculty of Pharmacy, University of Medicine and Pharmacy of Craiova, 2 Petru Rareş Street, 200349 Craiova, Dolj County, Romania

5. 5 Department of Pharmaceutical Botany, Faculty of Pharmacy, University of Medicine and Pharmacy of Craiova, 2 Petru Rareş Street, 200349 Craiova, Dolj County, Romania

6. 6 Department of Physical Medicine and Rehabilitation, Faculty of Medicine, University of Medicine and Pharmacy of Craiova, 2 Petru Rareş Street, 200349 Craiova, Dolj County, Romania

7. 7 Department of Biochemistry, Faculty of Pharmacy, University of Medicine and Pharmacy of Craiova2 Petru Rareş Street, 200349 Craiova, Dolj County, Romania

8. 8 Department of Physics, Faculty of Pharmacy, University of Medicine and Pharmacy of Craiova2 Petru Rareş Street, 200349 Craiova, Dolj County, Romania

Abstract

The identification and quantitation of nicotinamide riboside (NAR) and its main related compound (nicotinamide) were achieved using high-performance thin-layer chromatography (HPTLC)–ultraviolet (UV) densitometry with confirmation by online electrospray ionization (ESI)–mass spectrometry (MS). As the stationary phase, HPTLC Si 60 F254 glass plates were employed; the mobile phase was ethanol–1 M ammonium acetate–formic acid (7:1:0.1, v/v/v). No derivatization was applied, and UV densitometry was performed in the absorbance mode (270 nm). The method was validated by specificity, linearity, accuracy, precision, and robustness.

Publisher

Akademiai Kiado Zrt.

Subject

General Chemistry

Link

https://akjournals.com/downloadpdf/journals/1326/32/2/article-p128.xml

Reference36 articles.

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2. Nrt1 and Tna1-Independent Export of NAD+ Precursor Vitamins Promotes NAD+ Homeostasis and Allows Engineering of Vitamin Production

3. Discoveries of Nicotinamide Riboside as a Nutrient and Conserved NRK Genes Establish a Preiss-Handler Independent Route to NAD+ in Fungi and Humans

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