Affiliation:
1. 1 Institute of Animal Reproduction and Food Research of the Polish Academy of Sciences Division of Reproductive Endocrinology and Pathophysiology 10-747 Olsztyn Tuwima 10 Poland
2. 2 University of Warmia and Mazury Department of Human Physiology, Faculty of Medical Sciences Olsztyn Poland
3. 3 University of Warmia and Mazury Division of Clinical Physiology, Department of Functional Morphology, Faculty of Veterinary Medicine Olsztyn Poland
Abstract
The distribution and co-localisation patterns of dopamine-β-hydroxylase (DβH), neuropeptide Y (NPY), somatostatin (SOM) and galanin (GAL) were investigated by use of retrograde neuronal tracing and double-labelling immunofluorescence techniques in the caudal mesenteric ganglion (CaMG) neurons supplying the ovary of adult pigs. The existence and density of nerve fibres that are immunoreactive (IR) for the above-mentioned neuroactive substances were also evaluated. Injections of a fluorescent tracer (Fast Blue; FB) into the ovaries revealed the presence of small- (76.38%) and large-sized (23.62%) FB-positive postganglionic neurons in the CaMG. Noradrenergic FB-positive cells were simultaneously NPY- (43.38%), SOM- (18.77%) and GAL- (18.31%) IR. Of the examined FB-positive neurons, 53.49% were DβH-IR but NPY-immunonegative (IN), 79.06% were DβH-IR but SOM-IN, and 77.16% were DβH-IR but GAL-IN. Small- or large-sized subsets of traced neurons were supplied by only one or a few nerve fibres, exhibiting DβH-, NPY-, SOM- and/or GAL-IR. Our data show the specific morphological as well as immunochemical structural organisation of the sympathetic neurons in the CaMG in adult gilts. The occurrence of an abundant population of noradrenergic perikarya in the CaMG may suggest their important physiological role in the regulation of gonadal function(s) in these animals.
Reference32 articles.
1. Co-existence and origin of peptidergic and adrenergic nerves in the guinea pig uterus. Retrograde tracing and immunocytochemistry, effects of chemical sympathectomy, capsaicin treatment and pregnancy;Alm P.;Cell Tissue Res.,1988
2. Nitric oxide synthase-containing neurons in rat parasympathetic, sympathetic and sensory ganglia: a comparative study;Alm P.;Histochem. J.,1995
3. Enkephalin-like immunoreactive principal ganglion cells and nerve fibres in the inferior mesenteric ganglion of the cat;Balayadi M.;Neurosci. Lett.,1988
4. Maturational changes in sympathetic and sensory innervation of the rat uterus: effects of neonatal capsaicin treatment;Brauer M. M.;Int. J. Dev. Neurosci.,1994
5. Distribution of efferent neurons innervating the oviduct in the pig;Czaja K.;Folia Morphol.,2001
Cited by
6 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献