Affiliation:
1. 1 Corvinus University of Budapest Department of Microbiology and Biotechnology Somlói út 14–16 H-1118 Budapest Hungary
2. 2 Eötvös Loránd University Department of Plant Anatomy Pázmány Péter sétány 1/C H-1117 Budapest Hungary
Abstract
Scanning and transmission electron microscopic studies revealed the presence of slime-like, amorphous material on the surface of
Schizosaccahromyces pombe
RIVE 4-2-1 cells, independently, whether they were in flocculated or in non-flocculated state. Close contact of the adjacent cells via the merging outermost cell wall layers was found, however, only in the case of floc formation, which was induced by cultivating the cells in the presence of 6% (v/v) ethanol. Irreversible loss of the flocculation ability of the cells by treatment with proteinases suggests that proteinaceous cell surface molecules as lectins contribute to the cell-to-cell interaction during flocculation. Both proteinase K and pronase treatments removed a distinct outer layer of the cell wall, which indicated that the protein moieties of the phosphogalactomannan outer surface layer has a crucial role in the maintenance of cell wall integrity. In the case of lysing enzyme treatment the removal of the outermost layer was also observed as the first step of the cell wall digestion, while driselase treatment resulted in almost complete digestion of the cell wall.
Subject
General Immunology and Microbiology,General Medicine
Cited by
2 articles.
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