Application of real-time rt-pcr utilising lux (light upon extension) fluorogenic primer for the rapid detection of avian influenza viruses-Reference-Cited by-同舟云学术

Application of real-time rt-pcr utilising lux (light upon extension) fluorogenic primer for the rapid detection of avian influenza viruses

Published:2006-12-01 Issue:4 Volume:54 Page:525-533
ISSN:0236-6290
Container-title:Acta Veterinaria Hungarica
language:
Short-container-title:

Author:

Kiss I.¹,Germán P.²,Sámi L.³,Antal Márta⁴,Farkas T.⁵,Kardos G.⁶,Kecskeméti S.⁷,Dán Á.⁸,Belák S.⁹

Affiliation:

1. 1 Central Veterinary Institute, Institute of Debrecen H-4031 Debrecen, Bornemissza u. 3-7, Hungary

2. 2 Central Veterinary Institute, Institute of Debrecen H-4031 Debrecen, Bornemissza u. 3-7, Hungary

3. 3 Central Veterinary Institute, Institute of Debrecen H-4031 Debrecen, Bornemissza u. 3-7, Hungary

4. 4 Central Veterinary Institute, Institute of Debrecen H-4031 Debrecen, Bornemissza u. 3-7, Hungary

5. 5 Central Veterinary Institute, Institute of Debrecen H-4031 Debrecen, Bornemissza u. 3-7, Hungary

6. 6 Central Veterinary Institute, Institute of Debrecen H-4031 Debrecen, Bornemissza u. 3-7, Hungary

7. 7 Central Veterinary Institute, Institute of Debrecen H-4031 Debrecen, Bornemissza u. 3-7, Hungary

8. 8 Central Veterinary Institute Budapest, Hungary

9. 9 Departments of Virology, National Veterinary Institute and Swedish University of Agricultural Sciences Uppsala, Sweden

Abstract

A real-time RT-PCR assay utilising light upon extension fluorogenic primer (LUX RT-PCR) was developed for the rapid and efficient detection of avian influenza viruses (AIV). The assay detected each of the AIV isolates tested (16/16) and gave negative results with heterologous pathogens (17/17). The detection limit of the assay proved to be 10-0.5 EID50/0.2 ml and 101.5 EID50/0.2 ml in allantoic fluid of virus-infected embryonated chicken eggs and in spiked chicken faeces samples, respectively. Based on its specificity, sensitivity and relative simplicity, the LUX RT-PCR assay provides a novel, rapid and cost-effective diagnostic tool for avian influenza surveillance and monitoring programs.

Publisher

Akademiai Kiado Zrt.

Subject

General Veterinary

Link

https://akademiai.com/doi/pdf/10.1556/AVet.54.2006.4.10

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