Morphofunctional Rearrangement of the Fibrous Structures of the Rat Dermis under the Conditions of Implantation of 3D Scaffold Based on Polyprolactone

Abstract

Background: the use of various scaffolds allows us to model the future fibrous framework of the newly formed regenerate, and also serves as a substrate for the settlement of the cellular component. The development of tissue engineering in regenerative medicine demands an understanding of the more specific mechanisms of the formation of the connective framework at the site of the defect. The aim of this research was to study the morphofunctional rearrangement of the fibrous structures of the rat dermis in response to the implantation of a 3D scaffold based on polyprolactone Methods and Results: The experiment was performed on 30 white male Wistar rats. The object of the study was a skin fragment together with an implantable 3D scaffold based on polyprolactone, taken on Days 3, 7 and 14 after implantation. Biomaterial with implantable scaffold was studied using light and scanning electron microscopy. The results of the study indicate that the 3D scaffold based on polyprolactone has good biocompatibility, causing a weak inflammatory reaction, and contributes to the formation of the connective tissue framework by Day 14. Conclusion: The results of the study can be used to develop new scaffolds or modify existing ones, as a "framework" for populating the cellular component and creating tissue-engineering structures.