The Level of Certain Interleukins and Tissue Factor in Conditions of Intestinal Ischemia and Reperfusion in Rats

Abstract

Background: The aim of this study was to estimate the blood levels of interleukins (ILs) and tissue factor (TF) in rat blood when modeling acute mesenteric ischemia (AMI) in various stages and during reperfusion. Methods and Results: The study was performed on white non-linear male rats, weighing 200±25g. AMI was simulated by ligating the cranial mesenteric artery at the mesentery root. Then, at certain time intervals, a relaparotomy was performed, blood was collected, and the animals were subjected to reperfusion at different time intervals. During the experiment, the animals were divided into 3 groups depending on the time of ischemia (3 hours [n=10], 6 hours [n=10], and 8 hours [n=10]) followed by reperfusion after the indicated time of ischemia. The level of ILs (IL-6, IL-8, and IL-10) and TF in rat blood serum was determined by EIA. We found that AMI in rats is accompanied by pronounced changes in the immune system and the development of a systemic inflammatory reaction syndrome, which is aggravated by reperfusion. At the same time, the restoration of blood flow after 3-hour ischemia was characterized by an increase in the concentration of IL-6, IL-8, and IL-10; after 6-hour ischemia – by an increase in the IL-6, IL-8 content and TF, and stabilization of the IL-10 concentration. Reperfusion after 8-hour ischemia was accompanied by an increase in the IL-6 concentration, a decrease in the levels of IL-8, IL-10, and TF, which can lead to the progression of necrotic changes in the intestine. Conclusion: The detected changes can serve as laboratory markers that characterize the course and stage of acute mesenteric ischemia.