Calcium carbonate precipitation by cyanobacterial polysaccharides

Abstract

AbstractCyanobacteria have been recognized as key players in the precipitation of calcium carbonate in marine and freshwater systems. These bacteria increase pH, (as a result of photosynthetic activity) and also produce extracellular polysaccharides, which act as binding sites for Ca2+ and CO32−. Both processes influence the morphology and the mineralogy of the carbonate minerals. In order to clarify the role of polysaccharides of picocyanobacteria upon calcium carbonate precipitation, both their buffering capacity and ability to induce precipitation need to be investigated. In this experimental study, we characterized the polysaccharides of three unicellular autotrophic picocyanobacterial Synechococcus-type strains by potentiometric titration and infrared spectroscopy. Potentiometric titrations were conducted to determine the total buffering capacity. The nature and concentration of active sites of the polysaccharides was clarified with the aid of potentiometric titration and spectral analysis of an aqueous cellular suspension. Precipitation experiments with polysaccharides of different strains allowed an estimation of their potential to precipitate calcium carbonate. The results presented here indicate that polysaccharides from cyanobacteria have a strong potential to exchange protons with their surrounding environment. Precipitation experiments demonstrated that extracellular polysaccharides of all the strains studied able to precipitate calcium carbonate.