Amplified Immunohistochemical Detection of PrPsc in Animal Transmissible Spongiform Encephalopathies Using Streptomycin-Reference-Cited by-同舟云学术

Amplified Immunohistochemical Detection of PrPsc in Animal Transmissible Spongiform Encephalopathies Using Streptomycin

Published:2006-04-17 Issue:8 Volume:54 Page:849-853
ISSN:0022-1554
Container-title:Journal of Histochemistry & Cytochemistry
language:en
Short-container-title:J Histochem Cytochem.

Author:

Bencsik Anna A.¹,Coleman Anthony W.²,Debeer Sabine O.S.¹,Perron Hervé³,Moussa Aly¹

Affiliation:

1. Agence Française de Sécurité, Sanitaire des Aliments, Laboratoire d'Etudes et de Recherches en Pathologie Bovine et Hygiène des Viandes, Unité ATNC, Lyon, France

2. Institut de Biologie et de Chimie des Protéines, University Lyon 1, Lyon, France

3. BioMérieux, R&D Immunoassay and Proteomics Department, Marcy l'Etoile, France

Abstract

Due to its sensitivity, immunohistochemistry (IHC) of abnormal prion protein (PrPsc) is used to study experimental and natural cases of transmissible spongiform encephalopathies (TSEs) such as Creutzfeldt-Jakob disease in humans or scrapie and bovine spongiform encephalopathy (BSE) in animals. The limits of detection are particularly critical when PrPsc IHC is used for diagnostic purposes. In this article, we describe for the first time the use of streptomycin sulfate in IHC, providing a novel original and easy way to amplify specifically PrPsc immunohistochemical detection in natural cases of BSE and scrapie, as well as in experimental TSEs in mice models using two different PrP antibodies. (J Histochem Cytochem 54:849-853, 2006)

Publisher

SAGE Publications

Subject

Histology,Anatomy

Link

http://journals.sagepub.com/doi/pdf/10.1369/jhc.5C6895.2006

Reference12 articles.

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2. An Alternative Pretreatment Procedure in Animal Transmissible Spongiform Encephalopathies Diagnosis Using PrPsc Immunohistochemistry

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4. Identification of a second bovine amyloidotic spongiform encephalopathy: Molecular similarities with sporadic Creutzfeldt-Jakob disease

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