Fluorescence and Electron Microscopic Localization of F-actin in the Ependymocytes

Author:

Li Yan-Chao1,Bai Wan-Zhu1,Sakai Kazuhisa1,Hashikawa Tsutomu1

Affiliation:

1. Neural Architecture, Advanced Technology Development Group, RIKEN Brain Science Institute, Saitama, Japan

Abstract

The organization of F-actin in the ventricular system has been reported to display pronounced regional differences with respect to shape, size, and development. However, the real roles played by F-actin in these cells cannot be understood unless the precise localization of F-actin is defined. In the present study, we used double-fluorescence labeling to further examine the localization of F-actin in the ependymocytes and its spatial relation to the other two cytoskeletal components, microtubules and intermediate filaments. Then we converted fluorescence signals for F-actin to peroxidase/DAB reaction products by use of a phalloidin-based FITC-anti-FITC system. This detection technique provided an overview of the distribution of F-actin in the ependymocytes at the ultrastructural level, and has been proven to be helpful in correlating light and electron microscopic investigations.

Publisher

SAGE Publications

Subject

Histology,Anatomy

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