Cellular and Subcellular Aquaporin-4 Distribution in the Mouse Neurohypophysis and the Effects of Osmotic Stimulation

Author:

Mesbah-Benmessaoud Ouahiba123,Benabdesselam Roza123,Hardin-Pouzet Hélène123,Dorbani-Mamine Latifa123,Grange-Messent Valérie123

Affiliation:

1. Laboratoire de Neurochimie/LBPO, Bab Ezzouar, Alger, Algérie (OM-B, RB, LD-M)

2. Unité de Recherches, Faculté des Sciences Biologiques/UMMTO, Tizi-Ouzou, Algérie (RB)

3. Université Pierre et Marie Curie, UPMC-Paris VI, INSERM UMRS 952, CNRS UMR 7224, PMSNC, Paris, France (HH-P, VG-M)

Abstract

Water channel aquaporin-4 (AQP4) is the most abundant water channel in the rodent brain and is mainly expressed in cerebral areas involved in central osmoreception and osmoregulation. The neurohypophysis is the release site of hypothalamic neurohormones vasopressin and oxytocin, which are involved in the regulation of the water balance. The authors investigated the cellular and subcellular distribution of AQP4 in the mouse neurohypophysis before and after chronic osmotic stimulation, using immunofluorescence microscopy and immunoperoxidase electron microscopy. They showed that AQP4 was abundant in the mouse hypophysis, mainly in the neural lobe. AQP4 was discontinuously distributed along pituicytes plasma membranes, in the dense neurosecretory granules and microvesicles of nerve endings and fibers, and along the luminal and abluminal membranes of fenestrated capillary endothelial cells. After chronic osmotic stimulation, AQP4 immunolabeling was enhanced. Taken together, these results suggest that AQP4 could be involved in the pituicyte sensor effect during osmoregulation, the modification and/or maturation mechanism of neurosecretory granules during neurohormone release, and the blood perfusion of the hypophysis.

Publisher

SAGE Publications

Subject

Histology,Anatomy

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