A New Multicolor Fluorescence In Situ Hybridization Probe Set Directed Against Human Heterochromatin-Reference-Cited by-同舟云学术

A New Multicolor Fluorescence In Situ Hybridization Probe Set Directed Against Human Heterochromatin

Published:2012-04-17 Issue:7 Volume:60 Page:530-536
ISSN:0022-1554
Container-title:Journal of Histochemistry & Cytochemistry
language:en
Short-container-title:J Histochem Cytochem.

Author:

Bucksch Maria¹²³⁴⁵,Ziegler Monika¹²³⁴⁵,Kosayakova Nadezda¹²³⁴⁵,Mulatinho Milene V.¹²³⁴⁵,Llerena Juan C.¹²³⁴⁵,Morlot Susanne¹²³⁴⁵,Fischer Wolfgang¹²³⁴⁵,Polityko Anna D.¹²³⁴⁵,Kulpanovich Anna I.¹²³⁴⁵,Petersen Michael B.¹²³⁴⁵,Belitz Britta¹²³⁴⁵,Trifonov Vladimir¹²³⁴⁵,Weise Anja¹²³⁴⁵,Liehr Thomas¹²³⁴⁵,Hamid Ahmed B.¹²³⁴⁵

Affiliation:

1. Institute of Human Genetics, Jena University Hospital, Jena, Germany (MB,MZ,NK,AW,TL,ABH)

2. Medical Genetics Department, Fernandes Figueira Institute, FIOCRUZ, Rio de Janeiro, Brazil (MVM,JCL)

3. MVZ Wagnerstibbe, Hannover, Germany (SM,WF)

4. National Medical Center, Minsk, Belarus (ADP)

5. Belarus State Medical University, Minsk, Belarus (AIK)

Abstract

A new multicolor fluorescence in situ hybridization (mFISH) probe set is presented, and its possible applications are highlighted in 25 clinical cases. The so-called heterochromatin-M-FISH (HCM-FISH) probe set enables a one-step characterization of the large heterochromatic regions within the human genome. HCM-FISH closes a gap in the now available mFISH probe sets, as those do not normally cover the acrocentric short arms; the large pericentric regions of chromosomes 1, 9, and 16; as well as the band Yq12. Still, these regions can be involved in different kinds of chromosomal rearrangements such as translocations, insertions, inversions, amplifications, and marker chromosome formations. Here, examples are given for all these kinds of chromosomal aberrations, detected as constitutional rearrangements in clinical cases. Application perspectives of the probe set in tumors as well as in evolutionary cytogenetic studies are given.

Publisher

SAGE Publications

Subject

Histology,Anatomy

Link

http://journals.sagepub.com/doi/pdf/10.1369/0022155412441708

Reference42 articles.

1. Prenatal diagnosis of a familial interchromosomal insertion of Y chromosome heterochromatin

2. Familial transmission of a translocation Y/14

3. Prenatal diagnosis of interstitially satellited 6p

4. A normal birth following preimplantation genetic diagnosis by FISH determination in the carriers of der(15)t(Y;15)(Yq12;15p11) translocations: two case reports

Cited by 17 articles. 订阅此论文施引文献订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献

1. Molecular combing and its application in clinical settings;Molecular Cytogenetics;2022-11-16

2. First Comprehensive Characterization of Phayre’s Leaf-Monkey (Trachypithecus phayrei) Karyotype;Frontiers in Genetics;2022-03-11

3. Molecular Cytogenetic Analysis of One African and Five Asian Macaque Species Reveals Identical Karyotypes as in Mandrill;Current Genomics;2018-02-20

4. The Use of Molecular Cytogenetic Techniques for the Identification of Chromosomal Abnormalities;Chromosomal Abnormalities - A Hallmark Manifestation of Genomic Instability;2017-08-30

5. Heterochromatin-Directed mFISH (HCM-FISH);Springer Protocols Handbooks;2016-10-18