Mapping of Carboxypeptidase M in Normal Human Kidney and Renal Cell Carcinoma

Author:

Denis Catherine J.123,Van Acker Nathalie123,De Schepper Stefanie123,De Bie Martine123,Andries Luc123,Fransen Erik123,Hendriks Dirk123,Kockx Mark M.123,Lambeir Anne-Marie123

Affiliation:

1. Laboratory of Medical Biochemistry, University of Antwerp, Belgium (CD,AML,DH)

2. HistoGeneX nv, Antwerp, Belgium (NVA,SDS,MDB,LA,MK)

3. StatUa Center for Statistics, University of Antwerp, Belgium (EF)

Abstract

Although the kidney generally has been regarded as an excellent source of carboxypeptidase M (CPM), little is known about its renal-specific expression level and distribution. This study provides a detailed localization of CPM in healthy and diseased human kidneys. The results indicate a broad distribution of CPM along the renal tubular structures in the healthy kidney. CPM was identified at the parietal epithelium beneath the Bowman’s basement membrane and in glomerular mesangial cells. Capillaries, podocytes, and most interstitial cells were CPM negative. Tumor cells of renal cell carcinoma subtypes lose CPM expression upon dedifferentiation. Tissue microarray analysis demonstrated a correlation between low CPM expression and tumor cell type. CPM staining was intense on phagocytotic tumor-associated macrophages. Immunoreactive CPM was also detected in the tumor-associated vasculature. The absence of CPM in normal renal blood vessels points toward a role for CPM in angiogenesis. Coexistence of CPM and the epidermal growth factor receptor (EGFR) was detected in papillary renal cell carcinoma. However, the different subcellular localization of CPM and EGFR argues against an interaction between these h proteins. The description of the distribution of CPM in human kidney forms the foundation for further study of the (patho)physiological activities of CPM in the kidney.

Publisher

SAGE Publications

Subject

Histology,Anatomy

Cited by 1 articles. 订阅此论文施引文献 订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献

1. Kallikrein in the Interstitial Space;Protein Science;2016-08-04

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