Immunohistochemical Localization of Deleted in Malignant Brain Tumors 1 in Normal Human Tissues

Author:

Bathum Nexoe Anders1ORCID,Pedersen Andreas Arnholdt1,von Huth Sebastian123,Detlefsen Sönke4,Hansen Pernille Lund5,Holmskov Uffe1

Affiliation:

1. Cancer and Inflammation Research, Department of Molecular Medicine

2. Department of Clinical Research, Faculty of Health Sciences

3. University of Southern Denmark, Odense, Denmark, and Department of Infectious Diseases

4. Department of Pathology, Odense University Hospital, Odense, Denmark

5. Department of Clinical Research, Faculty of Health Sciences and Molecular Oncology

Abstract

Deleted in malignant brain tumors 1 (DMBT1) is part of the innate immune system and is expressed on mucosal surfaces in various tissues throughout the human body. However, to date, the localization of DMBT1 has not been investigated systematically and comprehensively in normal human tissues. In this study, we analyzed the mRNA expression of DMBT1 in human tissue by quantitative real-time PCR and examined its localization and distribution in the tissue by immunohistochemical staining using the monoclonal DMBT1 antibody HYB213-6. Anti-ovalbumin was used as an isotype control. The highest level of mRNA expression of DMBT1 was found in the small intestine, and the expression level was high throughout the luminal digestive tract. The expression of DMBT1 was especially high in the luminal digestive tract and salivary glands. The lowest expression level was found in the spleen. Immunohistochemical staining showed a high expression level of DMBT1 on mucosal surfaces throughout the body. There was a clear correlation between the mRNA expression and immunohistochemical expression of DMBT1 in the tissue. DMBT1 is strongly expressed on mucosal surfaces and in salivary glands

Publisher

SAGE Publications

Subject

Histology,Anatomy

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