Synthesis and Characterization of Supported Bioactive Lipid Membranes

Author:

Winger Theodore M.,Chaikof Elliot L.

Abstract

AbstractSingle and multicomponent membrane-mimetic surfaces of DPPC and synthetic lipid-peptide conjugates were formed on an alkylated glass surface by a process of vesicle fusion. Correlative atomic force microscopy and radiochemical titration techniques confirmed the generation of a single substrate supported monolayer and predictable deposition of defined concentrations of lipopeptide. Mixed systems were stable for periods exceeding 1 month if stored at room temperature in phosphate buffered saline.

Publisher

Springer Science and Business Media LLC

Subject

General Engineering

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