Comparative Evaluation of the Efficacy of Bromelain with Biodentine and Biodentine as Isolation Materials for Direct Pulp Capping: A Research Protocol

Abstract

Introduction: Direct Pulp Capping (DPC) aims to retain the pulp's health by sealing it against microbes and stimulating the formation of a dentin bridge at the exposure site. It has been proven that natural antimicrobial proteins, when used as therapeutics in conjunction with traditional chemotherapeutic agents, have increased efficacy and presumably diminished the incidence of chemoresistance. Hence, the efficacy of Bromelain with Biodentine and Biodentine as isolation materials for DPC will be evaluated and compared in the present study. The success and durability of DPC depend on the type of material used. Thus, the present study focuses on evaluating and considering a combination of materials for DPC that can be used in clinical practice. Need for the study: By addressing the limitations of current materials and formulating a novel combination, the present study aims to contribute to the advancement of material science, providing solutions that meet the demands of this era. Such a breakthrough would not only enhance the performance and longevity of materials but also have a significant positive impact on the preservation of pulp vitality, promoting overall well-being and improved outcomes in healthcare applications. Aim: To evaluate and compare the efficacy of Bromelain with Biodentine and Biodentine in isolation as DPC materials. Objectives: The study aims to evaluate and compare the setting time, flow, solubility, compressive strength, adhesion, and radiopacity of bromelain with biodentine and biodentine in isolation. Materials and Methods: An in-vitro study will be conducted in the Department of Conservative Dentistry and Endodontics at Sharad Pawar Dental College and Hospital, Wardha, Maharashtra, India, for a duration of nine months from May 2023 to February 2024. Standard discs of freshly mixed materials (n=10 samples per material) will be prepared. Compressive strength, solubility in distilled water, setting time, flow, radiopacity, and adhesion of Bromelain with Biodentine and Biodentine in isolation will be evaluated and compared in accordance with the International Standard ISO 6876:2001. Results will be analysed using Tukey’s Honest Significant Difference (HSD) posthoc test, one-way Analysis of Variance (ANOVA), and student’s t-test.