Comparison of Antimicrobial Efficacy of Garlic, Ginger, Cardamom Oil and Chlorhexidine against Streptococcus mutans: An In-vitro Study

Abstract

Introduction: Essential herbs such as garlic, ginger, and cardamom have shown antimicrobial activity with no potential adverse effects and are cost-effective. Despite these advantages, the efficacy of these agents needs to be tested against common oral pathogenic microorganisms to ensure that if found effective, they can be used in clinical settings. Aim: To evaluate and compare the effectiveness of garlic, ginger, and cardamom oils on Streptococcus mutans (S. mutans) against chlorhexidine. Materials and Methods: An in-vitro study was conducted at Bharati Vidyapeeth Dental College and Hospital, Navi Mumbai, in the Department of Microbiology and Bharati Vidyapeeth College of Pharmacy, Navi Mumbai, Maharashtra, India from January 2023 to September 2023. Oil extracts of garlic, ginger, and cardamom were loaded onto sterile filter paper discs measuring 6 mm in diameter in concentrations of 50 µL, 100 µL, and 200 µL. Additionally, 2% chlorhexidine (control group) was loaded onto similar sterile paper discs. The discs were dried and placed aseptically on culture media plates inoculated with S. mutans, and the plates were then incubated at 37°C overnight. Subsequently, the zones of inhibition were measured in millimeters. Results: Ginger oil exhibited the highest zone of inhibition, measuring 25 mm, followed by cardamom oil and garlic oil measuring 18 mm and 12 mm, respectively, at a concentration of 200 µL. The zone of inhibition measured for 100 µL concentrations of garlic, ginger, and cardamom oils were 7 mm, 16 mm, and 13 mm, respectively. For 50 µL concentrations of the oils, the zones of inhibition for garlic, ginger, and cardamom were 0 mm, 13 mm, and 9 mm, respectively. The zone of inhibition shown by chlorhexidine was 22 mm for concentrations of 50 µL, 100 µL, and 200 µL. Conclusion: Essential oil extracts from ginger, cardamom, and garlic were found to have antimicrobial activity against S. mutans, with ginger oil showing the highest effectiveness, followed by cardamom oil and garlic oil.