Anti-Inflammatory Effect of Acetone Extracts from Microalgae Chlorella sp. WZ13 on RAW264.7 Cells and TPA-induced Ear Edema in Mice

Author:

Yang Longhe,Hu Fan,Yan Yajun,Yu Siyu,Chen Tingting,Wang Zhaokai

Abstract

Microalgae extracts have a wide range of uses in the field of healthcare and nutrition. However, the use of microalgae extracts in anti-inflammatory properties and their mechanism of action have not yet been fully studied. Here, we show that extracts from Chlorella sp. WZ13 (CSE-WZ13) dose-dependently reduced nitrite production, inhibited the expression of inducible nitric oxide synthase (iNOS) protein, and decreased the production of the gene and inflammatory cytokines tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6), in lipopolysaccharide (LPS)-stimulated RAW264.7 cells. Using high-content imaging analysis, it was found that CSE-WZ13 inhibited the translocation of nuclear factor kappa B (NF-κB) from the cytoplasm to the nucleus. CSE-WZ13 also exerted anti-inflammatory effects in an ear edema mouse model induced by 12-O-tetradecanoylphorbol-13-acetate (TPA). CSE-WZ13 inhibited edema by 36.17% and 25.66% at a dose of 0.3 and 0.1 mg/ear, respectively. Histological analysis showed that topical application of CSE-WZ13 decreased TPA-induced inflammatory cell infiltration. Our results indicate that CSE-WZ13 may be a useful candidate for the purpose of decreasing inflammation.

Funder

National Natural Science Foundation of China

Natural Science Foundation of Fujian Province

Publisher

Frontiers Media SA

Subject

Ocean Engineering,Water Science and Technology,Aquatic Science,Global and Planetary Change,Oceanography

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