Dietary Arginine Modulates Growth Performance, Hemato-Biochemical Indices, Intestinal Enzymes, Antioxidant Ability and Gene Expression of TOR and 4E-BP1 in Rainbow Trout, Oncorhynchus mykiss Fingerlings

Author:

Ahmed Imtiaz,Ahmad Ishtiyaq,Malla Bashir Ahmad,Shah Basit Amin,Wani Zubair Ahmad,Khan Younis Mohd

Abstract

We evaluated the effect of arginine on growth, hemato-biochemical variables, intestinal enzymes, antioxidant ability, and expression of target of rapamycin (TOR) signaling pathway related genes in fingerling Oncorhynchus mykiss. A series of six uniform diets consisting of 450 g kg-1 of protein and 20.9 g-1 of energy with graduated levels (10.0, 12.5, 15.0, 17.5, 20.0, and 22.5 g kg-1) of arginine were formulated. Significant (p < 0.05) variations were observed in various analyzed parameters. Growth parameters showed a positive linear trend with increasing arginine levels up to 17.5 g kg-1. Fish offered 17.5 g kg-1 of dietary arginine exhibited highest body protein, lowest moisture, and intermediate fat contents, whereas, body ash content did not significantly (p > 0.05) change with respect to each dietary arginine offered diet, except for the lowest levels. Each group also produced significant (p < 0.05) changes in their hematological variables with maximum hemoglobin (Hb), hematocrit (Hct) content, and red blood cell (RBC) count noted at 17.5 g kg-1 arginine diet. Although the majority of blood plasma parameters were insignificantly (p > 0.05) different with respect to varied arginine fed diets, plasma aspartate transaminase (AST) showed significant (p < 0.05) variations across the treatments. Moreover, total protein, alkaline phosphatase (ALP) and albumin contents increased significantly (p < 0.05) with increasing arginine concentrations up to 17.5 g kg-1 and, thereafter, a declined trend in these parameters were noted. Intestinal enzymes and antioxidant properties showed significant (p < 0.05) variations, except plasma amylase, which showed insignificant variation. The maximum up-regulation of TOR and 4E-BP1 gene expression levels were found for fish fed 17.5 g kg-1 of dietary arginine compared to the lower (10.0 and 12.5 g kg-1) and higher (20.0 and 22.5 g kg-1) arginine levels, which indicates that fish utilize arginine more efficiently at this point. Quadratic regression analysis of live weight gain (LWG), feed conversion ratio (FCR), protein efficiency ratio (PER) and body protein deposition (BPD) indicated the optimal arginine requirement of rainbow trout to be 17.61 g kg-1 of dry diet, corresponding to 39.13 g kg-1 of dietary protein, which enhances growth, immunity, antioxidant ability, and also promote TOR signaling pathway in rainbow trout.

Funder

Department of Biotechnology, Ministry of Science and Technology, India

Publisher

Frontiers Media SA

Subject

Ocean Engineering,Water Science and Technology,Aquatic Science,Global and Planetary Change,Oceanography

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3