Author:
Tomita Taketeru,Toda Minoru,Murakumo Kiyomi,Kaneko Atsushi,Yano Nagisa,Nakamura Masaru,Sato Keiichi
Abstract
In this study, we developed an extra-uterine life-support system (artificial uterus) for viviparous elasmobranchs. Using this system, we maintained two embryonic specimens of the slendertail lantern shark (Etmopterus molleri) for approximately 5 months, the longest published record of the captive maintenance of the embryos of this species. This system is characterized by the use of a urea-containing artificial incubation fluid, of which the salinity and osmotic pressure are approximately equivalent to those of shark blood plasma. We hypothesized that this fluid reduces the salinity gradient between embryonic blood plasma and the environmental fluid, which thereby contributes to countering the low osmoregulatory capacity of viviparous shark embryos. However, the incubated specimens subsequently died during the process of seawater adaptation following “artificial birth.” Accordingly, information pertaining to uterine chemical dynamics throughout the gestation period is necessary for the design of a more effective incubation system. This technique will be relevant and may find application in conservation breeding at public aquaria in the future.
Funder
Japan Society for the Promotion of Science
Subject
Ocean Engineering,Water Science and Technology,Aquatic Science,Global and Planetary Change,Oceanography
Reference18 articles.
1. A series of normal stages for development of Scyliorhinus canicula, the lesser spotted dogfish (Chondrichthyes: Scyliorhinidae).;Ballard;J,1993
2. Sharks of the world. An annotated and illustrated catalogue of shark species known to date.;Compagno;FAO Fish. Synop.,1984
3. Reproduction and embryonic development in two species of squaliform sharks, Centrophorus granulosus and Etmopterus princeps: evidence of matrotrophy?;Cotton;Deep Sea Res,2015
4. Etmopterus spinax, the velvet belly lanternshark, does not use bacterial luminescence.;Duchatelet;Acta Histochem,2019
5. Uterine fluid composition of the dwarf ornate wobbegong shark (Orectolobus ornatus) during gestation.;Ellis;Mar,2011
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