Author:
Lin Yu-Hsien,Maaroufi Houda Ouns,Kucerova Lucie,Rouhova Lenka,Filip Tomas,Zurovec Michal
Abstract
Adenosine (Ado) is an important signaling molecule involved in stress responses. Studies in mammalian models have shown that Ado regulates signaling mechanisms involved in “danger-sensing” and tissue-protection. Yet, little is known about the role of Ado signaling inDrosophila. In the present study, we observed lower extracellular Ado concentration and suppressed expression of Ado transporters in flies expressing mutant huntingtin protein (mHTT). We altered Ado signaling using genetic tools and found that the overexpression of Ado metabolic enzymes, as well as the suppression of Ado receptor (AdoR) and transporters (ENTs), were able to minimize mHTT-induced mortality. We also identified the downstream targets of the AdoR pathway, the modifier of mdg4 (Mod(mdg4)) and heat-shock protein 70 (Hsp70), which modulated the formation of mHTT aggregates. Finally, we showed that a decrease in Ado signaling affects otherDrosophilastress reactions, including paraquat and heat-shock treatments. Our study provides important insights into how Ado regulates stress responses inDrosophila.
Subject
Cell Biology,Developmental Biology
Cited by
7 articles.
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