Author:
Wang Feifan,Zhang Yan,Zhou Xuejian,Chen Xianwu,Xiang Jiayong,Fan Mengjing,Yu Yanlan,Cai Yueshu,Wu Hongshen,Huang Shihan,He Ning,Hu Zhenghui,Ding Guoqing,Jin Xiaodong
Abstract
Circular RNA (circRNA) is a newly discovered endogenous non-coding RNA (ncRNA), which is characterized with a closed circular structure. A growing body of evidence has verified the vital roles of circRNAs in human cancer. In this research, we selected circPPP1CB as a study object by circRNA sequencing and quantitative real-time PCR (qRT-PCR) validation in human bladder cancer (BC). CircPPP1CB is downregulated in BC and is negatively correlated with clinical stages and histological grades. Functionally, circPPP1CB modulated cell growth, metastasis, and epithelial-to-mesenchymal transition (EMT) process in vitro and in vivo. Mechanically, we performed various experiments to verify the circPPP1CB/miR-1307-3p/SMG1 regulatory axis. Taken together, our results demonstrated that circPPP1CB participates in tumor growth, metastasis, and EMT process by interacting with the miR-1307-3p/SMG1 axis, and that circPPP1CB might be a novel therapeutic target and diagnostic biomarker in human BC.
Subject
Cell Biology,Developmental Biology
Reference57 articles.
1. Bladder cancer incidence and mortality: a global overview and recent trends.;Antoni;Eur. Urol.,2017
2. The landscape of microRNA, Piwi-interacting RNA, and circular RNA in human saliva.;Bahn;Clin. Chem.,2015
3. EMT, MET, plasticity, and tumor metastasis.;Bakir;Trends Cell Biol.,2020
4. Connecting the dots between different networks: miRNAs associated with bladder cancer risk and progression.;Braicu;J. Exp. Clin. Cancer Res.,2019
5. Exosomal MicroRNA-9-3p Secreted from BMSCs downregulates ESM1 to Suppress the Development of Bladder Cancer.;Cai;Mol. Ther. Nucleic Acids,2019
Cited by
9 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献