Effects of Three Types of Pollen on the Growth and Development of Honey Bee Larvae (Hymenoptera, Apidae)

Abstract

Pollen serves as an essential protein source for honey bee larvae. The nutrients in pollen greatly influence larval growth and development. Here, the survival, prepupal weight, developmental stage, pollen digestibility and midgut cells in honey bee (Apis mellifera L.) larvae were evaluated by performing in vitro and 5-ethynyl-2′-deoxyuridine (EdU) assays on larvae reared on three single pollens (Brassica napus L., Armeniaca sibirica L., and Pyrus bretschneideri Rehd.) and a pollen mixture (mixture of the three pollens in equal proportions). The results showed that the survival rate of larvae fed 10 mg of rape pollen was lowest (P < 0.05), but there were no notable differences in the survival rate among the groups receiving the other types and doses of pollen (P > 0.05). The prepupal weight of larvae fed apricot pollen was significantly lower than those of the other groups (P < 0.05). The digestibility of rape pollen and the pollen mixture were dramatically higher than those of apricot and pear pollen (P < 0.05). Pear and mixed pollen exerted negative effects on the nuclear area of midgut cells in the early larval stage (P < 0.05). In conclusion, detection of larval midgut cells using the EdU assay might be an effective method to assess the pollen nutritive value in honey bees. Compared to apricot and pear pollen, rape pollen was more beneficial in larval honey bee growth and development.