Population genetics of the naked mole-rat Heterocephalus glaber: The role of rivers in shaping genetic structure

Abstract

We used nested clade phylogeographic analysis (NCPA) of mitochondrial DNA sequence data to examine the processes contributing to population structure in naked mole-rats. We examined sequence variation in the (1097 bp) control region D-loop of the mitochondrial genome in 303 individuals from 174 colonies of naked mole-rats (Heterocephalus glaber) located mainly within an 870 km2 area in Meru National Park, Kenya. Four rivers were found to be correlated to a significant fragmentation inference in the NCPA. The largest pairwise divergence between haplotypes from populations separated by rivers was 2.74%, which was well over half of the divergence reported between the extremes of the distribution from southern Ethiopia to southern Kenya (4.6%). However, the size of the river (measured in current discharge) was not a good predictor of the amount of sequence divergence between populations separated by a river. It appears that a large-scale historical fragmentation event may have conflated fragmentation patterns on a smaller scale, when recent colonization and range expansion brought two old lineages together at a location with a relatively small river separating them.