Author:
Iwai Youichi,Ozawa Katsuya,Yahagi Kazuko,Mishima Tsuneko,Akther Sonam,Vo Camilla Trang,Lee Ashley Bomin,Tanaka Mika,Itohara Shigeyoshi,Hirase Hajime
Abstract
Astrocytes elicit transient Ca2+ elevations induced by G protein-coupled receptors (GPCRs), yet their role in vivo remains unknown. To address this, transgenic mice with astrocytic expression of the optogenetic Gq-type GPCR, Optoα1AR, were established, in which transient Ca2+ elevations similar to those in wild type mice were induced by brief blue light illumination. Activation of cortical astrocytes resulted in an adenosine A1 receptor-dependent inhibition of neuronal activity. Moreover, sensory stimulation with astrocytic activation induced long-term depression of sensory evoked response. At the behavioral level, repeated astrocytic activation in the anterior cortex gradually affected novel open field exploratory behavior, and remote memory was enhanced in a novel object recognition task. These effects were blocked by A1 receptor antagonism. Together, we demonstrate that GPCR-triggered Ca2+ elevation in cortical astrocytes has causal impacts on neuronal activity and behavior.
Funder
RIKEN
Japan Society for the Promotion of Science
Human Frontier Science Program
Novo Nordisk Fonden
Danmarks Frie Forskningsfond
Lundbeckfonden
Subject
Cellular and Molecular Neuroscience,Cognitive Neuroscience,Sensory Systems,Neuroscience (miscellaneous)
Cited by
32 articles.
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