Author:
Li Ye,Walker Logan A.,Zhao Yimeng,Edwards Erica M.,Michki Nigel S.,Cheng Hon Pong Jimmy,Ghazzi Marya,Chen Tiffany Y.,Chen Maggie,Roossien Douglas H.,Cai Dawen
Abstract
Identifying the cellular origins and mapping the dendritic and axonal arbors of neurons have been century old quests to understand the heterogeneity among these brain cells. Current Brainbow based transgenic animals take the advantage of multispectral labeling to differentiate neighboring cells or lineages, however, their applications are limited by the color capacity. To improve the analysis throughput, we designed Bitbow, a digital format of Brainbow which exponentially expands the color palette to provide tens of thousands of spectrally resolved unique labels. We generated transgenic Bitbow Drosophila lines, established statistical tools, and streamlined sample preparation, image processing, and data analysis pipelines to conveniently mapping neural lineages, studying neuronal morphology and revealing neural network patterns with unprecedented speed, scale, and resolution.
Funder
National Institute of Mental Health
National Institute of Neurological Disorders and Stroke
National Institute of Allergy and Infectious Diseases
Subject
Cellular and Molecular Neuroscience,Cognitive Neuroscience,Sensory Systems,Neuroscience (miscellaneous)
Cited by
5 articles.
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