Immobilisation of anaerobic digestate supplied nitrogen into soil microbial biomass is dependent on lability of high organic carbon materials additives

Abstract

Anaerobic digestate is a nutrient rich slurry by-product derived from biogas production, often used as a fertiliser due to its high nitrogen content. However, nitrogen losses from its application can lead to environmental pollution. In a laboratory experiment, the addition of high organic carbon materials to digestate-amended soil as a potential means to stimulate microbial immobilisation of digestate supplied nitrogen was investigated. Soil was incubated in pots for 5 months with digestate (equivalent to 250 kgN ha−1). The impact of adding carbon into the digestate (equivalent to 540 kgC ha−1) as either glycerol, straw, woodchip, or biochar on soil microbial and chemical parameters was quantified. Glycerol amended soils had significantly higher microbial biomass compared to digestate alone during the first month and at 30 days after application had a 4x higher on average microbial N. The digestate + straw treatment resulted in a 2.5x significantly greater nitrogen immobilisation compared to digestate alone after 3 months of incubation. The digestate + woodchip had a 2× higher mean microbial N after 5 months, whilst the biochar amendment did not stimulate significant nitrogen immobilisation at any time. These results suggest that mixing a labile to moderately labile organic carbon amendment, such as straw, with digestate has the greatest potential to reduce nitrogen losses following digestate application through microbial immobilisation.