Author:
Araújo Marília Ladeira de,Gomes Bruno Costa,Devóz Paula Pícoli,Duarte Nathália de Assis Aguilar,Ribeiro Diego Luis,Araújo Adriana Ladeira de,Batista Bruno Lemos,Antunes Lusânia Maria Greggi,Barbosa Fernando,Rodrigues António Sebastião,Rueff José,Barcelos Gustavo Rafael Mazzaron
Abstract
Experimental and epidemiologic studies have shown that lead (Pb) is able to induce epigenetic modifications, such as changes in DNA methylation profiles, in chromatin remodeling, as well as the expression of non-coding RNAs (ncRNAs). However, very little is known about the interactions between microRNAs (miRNAs) expression and DNA methylation status in individuals exposed to the metal. The aim of the present study was to investigate the impact of hsa-miR-148a expression on DNA methylation status, in 85 workers exposed to Pb. Blood and plasma lead levels (BLL and PLL, respectively) were determined by ICP-MS; expression of the miRNA-148a was quantified by RT-qPCR (TaqMan assay) and assessment of the global DNA methylation profile (by measurement of 5-methylcytosine; % 5-mC) was performed by ELISA. An inverse association was seen between miR-148a and % 5-mC DNA, as a function of BLL and PLL (β = −3.7; p = 0.071 and β = −4.1; p = 0.049, respectively) adjusted for age, BMI, smoking, and alcohol consumption. Taken together, our study provides further evidence concerning the interactions between DNA methylation profile and miR-148a, in individuals exposed to Pb.
Funder
Fundação para a Ciência e a Tecnologia
Fundação de Amparo à Pesquisa do Estado de São Paulo
Conselho Nacional de Desenvolvimento Científico e Tecnológico
Subject
Genetics(clinical),Genetics,Molecular Medicine
Cited by
11 articles.
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