Author:
Chen Limin,Ma Yamin,He Tianjun,Chen TingTing,Pan Yiming,Zhou Dayun,Li Xiaowei,Lu Yaobin,Wu Quancong,Wang Lailiang
Abstract
Sheath rot disease (SRD) is one of the most devastating diseases of Manchurian wild rice (MWR) (Zizania latifolia Griseb). Pilot experiments in our laboratory have shown that an MWR cultivar “Zhejiao NO.7”exhibits signs of SRD tolerance. To explore the responses of Zhejiao No. 7 to SRD infection, we used a combined transcriptome and metabolome analysis approach. A total of 136 differentially accumulated metabolites (DAMs, 114 up- and 22 down-accumulated in FA compared to CK) were detected. These up-accumulated metabolites were enriched in tryptophan metabolism, amino acid biosynthesis, flavonoids, and phytohormone signaling. Transcriptome sequencing results showed the differential expression of 11,280 genes (DEGs, 5,933 up-, and 5,347 downregulated in FA compared to CK). The genes expressed in tryptophan metabolism, amino acid biosynthesis, phytohormone biosynthesis and signaling, and reactive oxygen species homeostasis confirmed the metabolite results. In addition, genes related to the cell wall, carbohydrate metabolism, and plant-pathogen interaction (especially hypersensitive response) showed changes in expression in response to SRD infection. These results provide a basis for understanding the response mechanisms in MWR to FA attack that can be used for breeding SRD-tolerant MWR.
Subject
Genetics (clinical),Genetics,Molecular Medicine
Reference116 articles.
1. Metabolomic applications for understanding complex tripartite plant-microbes interactions: Strategies and perspectives;Adeniji;Biotechnol. Rep. (Amst),2020
2. ABA is an essential signal for plant resistance to pathogens affecting JA biosynthesis and the activation of defenses in Arabidopsis;Adie;Plant Cell.,2007
3. Recent advances in bio-chemical, molecular and physiological aspects of membrane lipid derivatives in plant pathology;Adigun;Plant, Cell. and Environ.,2021
4. Domestication of crop metabolomes: Desired and unintended consequences;Alseekh;Trends Plant Sci.,2021
5. Pathogenesis-related genes of PR1, PR2, PR4, and PR5 families are involved in the response to fusarium infection in garlic (Allium sativum L);Anisimova;Int. J. Mol. Sci.,2021