Author:
Zhang Qi-Qiang,Chen Feng-Hua,Wang Fei,Di Xue-Mei,Li Wei,Zhang Hai
Abstract
The monoester alkaloids in Aconitum carmichaelii, including benzoylaconitine (BAC), benzoylmesaconine, and benzoylhypaconitine, were found to have anti-hypertensive effects in spontaneously hypertension rats (SHRs), of which BAC is the strongest. However, its antihypertensive target and underlying molecular mechanisms remain unclear. In this study, first, we screened the antihypertensive targets of BAC by using the CVDPlatform (www.cbligand.org/CVD) and found that ACE/ACE2 are the most possible targets. Then, we verified the effect of BAC on ACE/ACE2 by virtual docking, SPR, enzyme activity assay, and HUVECs cell experiment. We found that BAC could bind with ACE/ACE2, inhibit ACE activity and protein expression, and activate ACE2 enzyme activity. Using vascular function test in vitro, we found that BAC could target ACE/ACE2 to enhance endothelium-dependent vasorelaxation. In BAC-treated SHRs, the levels of ACE and AngII in serum were reduced while Ang (1–7) was increased significantly, and the expression of ACE was reduced, which suggested that BAC can inhibit ACE and activate ACE2 to inhibit AngI to AngII and promote AngII to Ang (1–7) to inhibit vasoconstriction and finally attenuate hypertension. Furthermore, the signaling pathways with regard to vasorelaxation and vascular inflammation were investigated. The results showed that BAC could significantly activate Akt/eNOS, increase NO production, and promote endothelial-related vasodilation; BAC could also reduce inflammatory factors TNF-α and IL6, inhibition of COX-2 expression, and IKB-α phosphorylation to reduce vascular inflammation in SHRs. In brief, BAC targets ACE/ACE2 to enhance endothelium-dependent vasorelaxation and reduce vascular inflammation to attenuate hypertension as a potential modulator of the renin–angiotensin system.
Funder
National Natural Science Foundation of China
Natural Science Foundation of Shanghai
Subject
Pharmacology (medical),Pharmacology
Reference32 articles.
1. The Meaning of Mas;Bader;Hypertension,2018
2. Essential Role of Nitric Oxide in VEGF-Induced, Asthma-like Angiogenic, Inflammatory, Mucus, and Physiologic Responses in the Lung;Bhandari;Proc. Natl. Acad. Sci. U S A.,2006
3. ACE2, a New Regulator of the Renin-Angiotensin System;Burrell;Trends Endocrinol. Metab.,2004
4. World Hypertension Day and Global Awareness;Chockalingam;Can. J. Cardiol.,2008
5. Angiotensin-converting Enzyme 2 Is an Essential Regulator of Heart Function;Crackower;Nature,2002
Cited by
7 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献