Author:
Zhao Chenpei,Chen Huan,Liang Hao,Zhao Xiaoyu,Tang Wenli,Wei Maolian,Li Youzhi,Zhang Jianlong,Yu Xin,Chen Guozhong,Zhu Hongwei,Jiang Linlin,Zhang Xingxiao
Abstract
Lactobacillus plantarumcan interact with macrophages against bacterial enteropathy due to its potential ability to modulate macrophage polarization. However, this mechanism is not completely understood. TLR2 can recognize microbial components and trigger macrophage cytokine responses to different gram-positive strains. The aim of this study was to investigate whether probioticLactobacillus plantarumRS-09 can induce macrophage polarization againstSalmonellaTyphimurium infection via TLR2 signalling. BALB/c mice were preadministered RS-09 continuously for 7 days and then infected withSalmonellaTyphimurium ATCC14028. Mouse RAW264.7 mononuclear macrophages were stimulated with RS-09 and coincubated with ATCC14028 or PBS controls. The results of thein vivostudy indicated that RS-09 could relieveS. Typhimurium-induced splenomegaly, body weight loss and death rate. RS-09 also limited the colonization and translocation ofS. Typhimurium in the gastrointestinal tract and thereby protected against infection. We also observed that RS-09 upregulated the production of M1 macrophage characteristics (e.g., CD11c and IL-6) againstS. Typhimurium. Furthermore, RS-09 induced the expression of TLR2 in macrophages. In anin vitrostudy, treatment of RAW264.7 cells with RS-09 either concurrently with or beforeS. Typhimurium challenge enhanced the secretion of Reactive oxygen species and Nitric oxide. This effect was related to TLR2 and NF-κB activation. Based on these findings,Lactobacillus plantarumRS-09 was shown to modulate M1 macrophage polarization and induce TLR2-linked NF-κB signalling activity in the innate immune response toS. Typhimurium infection.
Subject
Pharmacology (medical),Pharmacology
Cited by
5 articles.
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