Author:
Pereira Inês,Lopez-Martinez Maria J.,Villasante Aranzazu,Introna Clelia,Tornero Daniel,Canals Josep M.,Samitier Josep
Abstract
Introduction: Three-dimensional (3D) bioprinting is a promising technique for the development of neuronal in vitro models because it controls the deposition of materials and cells. Finding a biomaterial that supports neural differentiation in vitro while ensuring compatibility with the technique of 3D bioprinting of a self-standing construct is a challenge.Methods: In this study, gelatin methacryloyl (GelMA), methacrylated alginate (AlgMA), and hyaluronic acid (HA) were examined by exploiting their biocompatibility and tunable mechanical properties to resemble the extracellular matrix (ECM) and to create a suitable material for printing neural progenitor cells (NPCs), supporting their long-term differentiation. NPCs were printed and differentiated for up to 15 days, and cell viability and neuronal differentiation markers were assessed throughout the culture.Results and Discussion: This composite biomaterial presented the desired physical properties to mimic the ECM of the brain with high water intake, low stiffness, and slow degradation while allowing the printing of defined structures. The viability rates were maintained at approximately 80% at all time points. However, the levels of β-III tubulin marker increased over time, demonstrating the compatibility of this biomaterial with neuronal cell culture and differentiation. Furthermore, these cells showed increased maturation with corresponding functional properties, which was also demonstrated by the formation of a neuronal network that was observed by recording spontaneous activity via Ca2+ imaging.
Funder
Centro de Investigación Biomédica en Red en Bioingeniería, Biomateriales y Nanomedicina
Instituto de Salud Carlos III
Ministerio de Ciencia, Innovación y Universidades
Direcció General de Recerca, Generalitat de Catalunya
European Commission
Subject
Biomedical Engineering,Histology,Bioengineering,Biotechnology
Cited by
4 articles.
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