Author:
Wongmoon Chanakarn,Napathorn Suchada Chanprateep
Abstract
In this study, a simple non-toxic recovery process of biodegradable poly(3-hydroxybutyrate) (PHB) using the green solvent 1,3-dioxolane and water was successfully developed. The critical parameters were optimized, and the process platform was scaled up from 2 ml to 1,000 ml for the efficient recovery of PHB. The physical parameters including continuous shaking, ultrasonication, extraction using the Soxhlet extractor, diluted 1,3-dioxolane, reused 1,3-dioxolane, and cell rupture by steam explosion prior to solvent extraction were carefully investigated. The results showed that continuous shaking played a major role in increasing the recovery efficiency during the scale-up process. The PHB extraction at 2 ml from dried cells at 80°C with 100 rpm of shaking speed for 5 h resulted in a recovery yield of 96.6 ± 0.1% with purity up to 99.1 ± 0.6% and that from wet cells under the same condition resulted in a recovery yield of 94.6 ± 4.8% and purity of 97.0 ± 0.1%. It should be noted that the PHB extracted from wet cells at room temperature with 150 rpm of shaking speed for 36 h resulted in a recovery yield of 93.5 ± 0.7% and purity of 97.7 ± 1.3% and had an MW of 3.1×105, MN of 2.7×105, and polydispersity index of 1.1. The direct scale-up process at 1,000 ml showed comparable results in purity, recovery yield, molecular weight distribution, thermal properties, and mechanical properties. The PHB extraction from dried cells gave the highest purity of 99.3 ± 0.5% and recovery of 94.0 ± 0.3%, whereas the PHB extraction from wet cells gave a purity of 90.3 ± 1.5% and recovery of 92.6 ± 1.0%. The novel recovery process showed its feasibility to be applied on an industrial scale.
Subject
Biomedical Engineering,Histology,Bioengineering,Biotechnology
Cited by
3 articles.
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