Author:
Zheng Judun,Yue Rui,Yang Ronghua,Wu Qikang,Wu Yunxia,Huang Mingxing,Chen Xu,Lin Weiqiang,Huang Jialin,Chen Xiaodong,Jiang Yideng,Yang Bin,Liao Yuhui
Abstract
Zika virus (ZIKV) is a re-emerging flavivirus that leads to devastating consequences for fetal development. It is crucial to visualize the pathogenicity activities of ZIKV ranging from infection pathways to immunity processes, but the accurate labeling of ZIKV remains challenging due to the lack of a reliable labeling technique. We introduce the photo-activated bio-orthogonal cycloaddition to construct a fluorogenic probe for the labeling and visualizing of ZIKV. Via a simple UV photoirradiation, the fluorogenic probes could be effectively labeled on the ZIKV. We demonstrated that it can be used for investigating the interaction between ZIKV and diverse cells and avoiding the autofluorescence phenomenon in traditional immunofluorescence assay. Thus, this bioorthogonal-enabled labeling strategy can serve as a promising approach to monitor and understand the interaction between the ZIKV and host cells.
Subject
Biomedical Engineering,Histology,Bioengineering,Biotechnology
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