Author:
Zheng Xiangjiao,Dong Zhiqiang,Liang Zepeng,Liu Yu,Yin Xiaowei,Han Mofei,Cui Zhongcheng,Mei Xifan,Gao Xiuqiu
Abstract
Introduction: Periodontitis is a chronic inflammatory disease brought on by various bacteria, and effective antibacterial, anti-inflammatory and alveolar bone regeneration are the main goals of treating periodontal disease.Methods: In the current work, we employed Icariin (ICA) into a hydrogel modified with carbon nanofiber (CNF) to create a multifunctional composite nanoplatform. The composite was activated in the near infrared (NIR) to treat periodontitis.Results: The antibacterial results showed that the ICA+CNF@H showed 94.2% and 91.7% clearance of S. aureus and E. coli, respectively, under NIR irradiation. In vitro experiments showed that NIR-irradiated composites suppressed inflammatory factor (IL-6) and ROS expression and up-regulated the performance of anti-inflammatory factor (IL-10) in RAW264.7 cells. At the same time, the composites promoted the production of osteogenic factors in BMSCs, with an approximately 3-fold increase in alkaline phosphatase activity after 7 days and an approximately 2-fold increase in the rate of extracellular matrix mineralization after 21 days. In vivo tests showed that the alveolar bone height was clearly greater in the ICA+CNF@H (NIR) group compared to the periodontitis group.Discussion: In conclusion, ICA+CNF@H under NIR irradiation achieved a synergistic effect of antibacterial, anti-inflammatory, reduction of reactive oxygen species and promotion of osteogenesis, offering a novel approach for treating periodontitis.
Funder
Natural Science Foundation of Liaoning Province
Subject
Biomedical Engineering,Histology,Bioengineering,Biotechnology
Reference47 articles.
1. Phosphorescent NIR emitters for biomedicine: Applications, advances and challenges;Chelushkin;Dalton Trans.,2022
2. An update on the progress of isolation, culture, storage, and clinical application of human bone marrow mesenchymal stem/stromal cells;Chu;Int. J. Mol. Sci.,2020
3. NIR-triggered tea polyphenol-modified gold nanoparticles-loaded hydrogel treats periodontitis by inhibiting bacteria and inducing bone regeneration;Dong;Mater. Des.,2023
4. Cell death in photodynamic therapy: From oxidative stress to anti-tumor immunity;Donohoe;Biochimica Biophysica Acta (BBA)-Reviews Cancer,2019
5. Icariin, an anti-atherosclerotic drug from Chinese medicinal herb horny goat weed;Fang;Front. Pharmacol.,2017