Gold Nanoparticle-Functionalized Diatom Biosilica as Label-Free Biosensor for Biomolecule Detection

Author:

Chen Tongtong,Wu Feifei,Li Yang,Rozan Hussein E.,Chen Xiguang,Feng Chao

Abstract

Diatom biosilica (DBs) is the cell wall of natural diatom called frustule, which is made of porous hydrogenated amorphous silica possessing periodic micro- to nanoscale features. In this study, a simple, sensitive, and label-free photoluminescence (PL) immune-detection platform based on functionalized diatom frustules was developed. Gold nanoparticles (AuNPs) deposited on poly-dopamine-coated diatom frustules via in situ deposition which considerably decreased the intrinsic blue PL intensity of diatom biosilica. Then, goat anti-rabbit immunoglobulin G (IgG) was added to functionalize diatom biosilica-poly-dopamine-AuNPs (DBs-PDA-AuNPs). PL studies revealed that the specific binding with antigen rabbit IgG increased the peak intensity of PL in comparison with the non-complimentary antigen (human IgG). The enhancement in PL intensity of DBs-PDA had a linear correlation with antigen (rabbit IgG) concentration, whose limit of detection (LOD) reached 8 × 10-6 mg/ml. Furthermore, PL detection based on DBs-PDA-AuNPs showed a high detection sensitivity with the LOD as low as 8 × 10-9 mg/ml and spread over almost eight orders of magnitude, making it suitable for the sensitive quantitative analysis of immune complex compared with traditional fluorescence immunoassay. Hence, the study proves that the AuNP-functionalized diatom frustules can serve as an effective biosensor platform for label-free PL-based immunoassay.

Funder

National Natural Science Foundation of China

Natural Science Foundation of Shandong Province

Publisher

Frontiers Media SA

Subject

Biomedical Engineering,Histology,Bioengineering,Biotechnology

Reference31 articles.

1. Photoluminescence Shift in Frustules of Two Pennate Diatoms and Nanostructural Changes to Their Pores;Arteaga-Larios;Luminescence,2014

2. Marine Diatoms as Optical Biosensors;De Stefano;Biosens. Bioelectron.,2009

3. Light Manipulation by Single Cells: The Case of Diatoms;De Tommasi;J. Spectrosc.,2016

4. Optics With Diatoms: Towards Efficient, Bioinspired Photonic Devices at the Micro-scale;De Tommasi,2013

5. Light Harvesting Proteins Regulate Non-photochemical Fluorescence Quenching in the Marine Diatom Thalassiosira Pseudonana;Dong;Algal Res.,2015

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3