Author:
Cheng Junrui,Balbuena Emilio,Miller Baxter,Eroglu Abdulkerim
Abstract
Background: Carotenoids are naturally occurring pigments accounting for the brilliant colors of fruits and vegetables. They may display antioxidant and anti-inflammatory properties in humans besides being precursors to vitamin A. There is a gap of knowledge in examining their role within colonic epithelial cells. We proposed to address this research gap by examining the effects of a major dietary carotenoid, β-carotene, in the in vitro epithelial cell model.Methods: We examined the function of β-carotene in the lipopolysaccharide (LPS)/toll-like receptor 4 (TLR4) signaling pathway. We conducted western blotting assays to evaluate expressions of TLR4 and its co-receptor, CD14. We also examined NF-κB p65 subunit protein levels in the model system. Furthermore, we studied the impact of β-carotene on the tight junction proteins, claudin-1, and occludin. We further carried out immunocytochemistry experiments to detect and visualize claudin-1 expression.Results: β-Carotene reduced LPS-induced intestinal inflammation in colonic epithelial cells. β-Carotene also promoted the levels of tight junction proteins, which might lead to enhanced barrier function.Conclusions: β-Carotene could play a role in modulating the LPS-induced TLR4 signaling pathway and in enhancing tight junction proteins. The findings will shed light on the role of β-carotene in colonic inflammation and also potentially in metabolic disorders since higher levels of LPS might induce features of metabolic diseases.
Subject
Nutrition and Dietetics,Endocrinology, Diabetes and Metabolism,Food Science
Reference64 articles.
1. Carotenoids: biochemistry, pharmacology and treatment;Milani;Br J Pharmacol.,2017
2. The promising effects of astaxanthin on lung diseases;Cheng;Adv Nutr,2020
3. Carotenoids database: structures, chemical fingerprints and distribution among organisms;Yabuzaki;Database.,2017
4. A global perspective on carotenoids: metabolism, biotechnology, and benefits for nutrition and health;Rodriguez-Concepcion;Prog Lipid Res.,2018
5. Carotenoids: a brief overview on its structure, biosynthesis, synthesis, and applications;Fernandes,2018
Cited by
24 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献