Leaf morphology, optical characteristics and phytochemical traits of butterhead lettuce affected by increasing the far-red photon flux

Abstract

Light and its spectral characteristics are crucial for plant growth and development. The far-red photon flux mediates many plant processes through the action of phytochrome and also accelerates the photosynthetic electron transfer rate. In this study, we assessed the effects of far-red addition on butterhead lettuce morphology, light use efficiency, optical properties, and phytochemical characteristics. Three-week-old lettuce plants (Lactuca sativa L. cv. Alyssa) were grown for up to 28 days under a 10% blue and 90% red light spectrum (200 µmol m-2 s-1, 16 h photoperiod) to which five different intensities of far-red light (peak at 735 nm) were added (0-9-18-36-72 µmol m-2 s-1). White light-emitting diodes were included as a proxy for sunlight. Increasing supplemental far-red photon flux from zero to 21% increased the light use efficiency (g per mol) by 37% on day 14; 43% on day 21; and 39% on day 28. Measurements of projected head area suggest that this was associated with an increase in leaf expansion and photon capture and not necessarily a direct effect on photosynthesis. Moreover, vegetation indices based on leaf reflectance showed a decrease in chlorophyll-related indices under a high far-red photon flux. This decrease in pigment content was confirmed by chemical analyses, suggesting that the plants may not reach their full potential in terms of photon capture, limiting the overall photosynthetic performance. Furthermore, the stress-related Carter 1 index increased in plants grown under a high far-red photon flux, indicating early plant stress. Far-red tended to decrease the content of total phenolics and increase soluble sugars. The higher sugar levels can be attributed to an improved photochemical efficiency due to photosystem I excitation by far-red wavelengths, also known as the Emerson Enhancement effect. Despite these higher sugar levels, no effect on foliar nitrate content was observed. Our results show that far-red supplementation has the potential to enhance light interception at the early growth stages, although higher intensities of far-red may cause plant stress.