Physiological Analysis and Transcriptome Sequencing Reveal the Effects of Salt Stress on Banana (Musa acuminata cv. BD) Leaf

Abstract

The salinization of soil is a widespread environmental problem. Banana (Musa acuminata L.) is a salt-sensitive plant whose growth, development, and production are constrained by salt stresses. However, the tolerance mechanism of this salt-sensitive banana to salt stress is still unclear. This study aimed to investigate the influence of NaCl treatment on phenotypic, physiological, and transcriptome changes in bananas. We found that the content of root activity, MDA, Pro, soluble sugar, soluble protein, and antioxidant enzymes activity in salt-stress treatment were significantly higher than the control in bananas. Transcriptome sequencing result identified an overall of 3,378 differentially expressed genes (DEGs) in banana leaves, and the Kyoto Encyclopedia of Genes and Genomes analysis indicated that these DEGs were involved in phenylpropanoid biosynthesis process, ribosome process, starch and sucrose metabolism, amino sugar process, and plant hormone signal transduction process that had simultaneously changed their expression under salt stress, which indicated these DEGs may play a role in promoting BD banana growth under salt treatments. The genes which were enriched in the phenylpropanoid biosynthesis process, starch and sucrose metabolism process, amino sugar process, and plant hormone signal transduction process were specifically regulated to respond to the salt stress treatments. Here, totally 48 differentially expressed transcription factors (TFs), including WRKY, MYB, NAC, and bHLH, were annotated in BD banana under salt stress. In the phenylpropane biosynthesis pathway, all transcripts encoding key enzymes were found to be significantly up-regulated, indicating that the genes in these pathways may play a significant function in the response of BD banana to salt stress. In conclusion, this study provides new insights into the mechanism of banana tolerance to salt stress, which provides a potential application for the genetic improvement of banana with salt tolerance.