Open-source software for automated rodent behavioral analysis

Abstract

Rodent behavioral analysis is a major specialization in experimental psychology and behavioral neuroscience. Rodents display a wide range of species-specific behaviors, not only in their natural habitats but also under behavioral testing in controlled laboratory conditions. Detecting and categorizing these different kinds of behavior in a consistent way is a challenging task. Observing and analyzing rodent behaviors manually limits the reproducibility and replicability of the analyses due to potentially low inter-rater reliability. The advancement and accessibility of object tracking and pose estimation technologies led to several open-source artificial intelligence (AI) tools that utilize various algorithms for rodent behavioral analysis. These software provide high consistency compared to manual methods, and offer more flexibility than commercial systems by allowing custom-purpose modifications for specific research needs. Open-source software reviewed in this paper offer automated or semi-automated methods for detecting and categorizing rodent behaviors by using hand-coded heuristics, machine learning, or neural networks. The underlying algorithms show key differences in their internal dynamics, interfaces, user-friendliness, and the variety of their outputs. This work reviews the algorithms, capability, functionality, features and software properties of open-source behavioral analysis tools, and discusses how this emergent technology facilitates behavioral quantification in rodent research.