Investigation of the Molecular Mechanisms by Which Endothelin-3 Stimulates Preadipocyte Growth

Author:

Siao An-Ci,Shih Li-Jane,Lin Yen-Yue,Tsuei Yi-Wei,Kuo Yow-Chii,Ku Hui-Chen,Chuu Chih-Ping,Hsiao Po-Jen,Kao Yung-Hsi

Abstract

Endothelins induce many biological responses, and they are composed of three peptides: ET-1, ET-2, and ET-3. Reports have indicated that ET-1 regulates cell proliferation, adipogenesis, and other cell responses and that ET-3 stimulates the growth of gastrointestinal epithelial cells and melanocytes. However, the signalling pathways of ET3 that mediate the growth of fat cells are still unclear. Using 3T3-L1 white preadipocytes, we found that ET-3 induced increases in both cell number and BrdU incorporation. Pretreatment with an ETAR antagonist (but not an ETBR antagonist) blocked the ET-3-induced increases in both cell number and BrdU incorporation. Additionally, BQ610 suppressed the ET-3-induced increases in phosphorylation of AMPK, c-JUN, and STAT3 proteins, and pretreatment with specific inhibitors of AMPK, JNK/c-JUN, or JAK/STAT3 prevented the ET-3-induced increases in phosphorylation of AMPK, c-JUN, and STAT3, respectively. Neither p38 MAPK inhibitor nor PKC inhibitor altered the effects of ET-3 on cell growth. These data suggest that ET-3 stimulates preadipocyte growth through the ETAR, AMPK, JNK/c-JUN, and STAT3 pathways. Moreover, ET-3 did not alter HIB1B brown preadipocyte and D12 beige preadipocyte growth, suggesting a preadipocyte type-dependent effect. The results of this study may help explain how endothelin mediates fat cell activity and fat cell-associated diseases.

Publisher

Frontiers Media SA

Subject

Endocrinology, Diabetes and Metabolism

Reference53 articles.

1. The Role of the Nrf2 Signaling in Obesity and Insulin Resistance;Li;Int J Mol Sci,2020

2. Endothelin;Davenport;Pharmacol Rev,2016

3. Endothelins: Molecular Biology, Biochemistry, Pharmacology, Physiology, and Pathophysiology;Rubanyi;Pharmacol Rev,1994

4. Obesity: The Disease of the Twenty-First Century;Rössner;Int J Obes,2002

5. Endothelin: 20 Years From Discovery to therapyThis Article is One of a Selection of Papers Published in the Special Issue (Part 2 of 2) on Forefronts in Endothelin;Barton;Can J Physiol Pharmacol,2008

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