Author:
Horii Tomomi,Kozawa Junji,Fujita Yukari,Kawata Satoshi,Ozawa Harutoshi,Ishibashi Chisaki,Yoneda Sho,Nammo Takao,Miyagawa Jun-ichiro,Eguchi Hidetoshi,Shimomura Iichiro
Abstract
Background and objectivePancreatic fat is a form of ectopic fat. Lipid droplets (LDs) are also observed in β cells; however, the pathophysiological significance, especially for β cell function, has not been elucidated. Our aim was to assess LD accumulation in β cells in various stages of glucose intolerance and to clarify its relationship with clinical and histological parameters.MethodsWe examined 42 Japanese patients who underwent pancreatectomy. The BODIPY493/503-positive (BODIPY-positive) area in β cells was measured in pancreatic sections from 32 patients. The insulin granule numbers were counted in an additional 10 patients using electron microscopy.ResultsThe BODIPY-positive area in β cells in preexisting type 2 diabetes patients was higher than that in normal glucose tolerance patients (p = 0.031). The BODIPY-positive area in β cells was positively correlated with age (r = 0.45, p = 0.0097), HbA1c (r = 0.38, p = 0.0302), fasting plasma glucose (r = 0.37, p = 0.045), and homeostasis model assessment insulin resistance (r = 0.41, p = 0.049) and negatively correlated with an increase in the C-peptide immunoreactivity level by the glucagon test (r = -0.59, p = 0.018). The ratio of mature insulin granule number to total insulin granule number was reduced in the patients with rich LD accumulation in β cells (p = 0.039).ConclusionsType 2 diabetes patients had high LD accumulation in β cells, which was associated with insulin resistance, hyperglycemia, aging and β cell dysfunction involving decreased mature insulin granules.
Funder
Japan Society for the Promotion of Science
Subject
Endocrinology, Diabetes and Metabolism
Reference44 articles.
1. Diagnosis and classification of diabetes mellitus;Diabetes Care,2014
2. Recent insights into mechanisms of β-cell lipo- and glucolipotoxicity in type 2 diabetes;Lytrivi;J Mol Biol,2020
3. Differential effects of monounsaturated, polyunsaturated and saturated fat ingestion on glucose-stimulated insulin secretion, sensitivity and clearance in overweight and obese, non-diabetic humans;Xiao;Diabetologia,2006
4. Nutrient-induced metabolic stress, adaptation, detoxification, and toxicity in the pancreatic β-cell;Prentki;Diabetes,2020
5. Lipomatosis of the pancreas in autopsy material and its relation to age and overweight;Olsen;Acta Pathol Microbiol Scand,1978
Cited by
10 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献