Chronic alcohol administration alters metabolomic profile of murine bone marrow
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Published:2023-04-05
Issue:
Volume:14
Page:
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ISSN:1664-3224
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Container-title:Frontiers in Immunology
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language:
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Short-container-title:Front. Immunol.
Author:
Pontes Pereira Tássia Tatiane,Fideles Duarte-Andrade Filipe,Gardone Vitório Jéssica,do Espírito Santo Pereira Taiane,Braga Martins Flavia Rayssa,Marques Souza Jéssica Amanda,Malacco Nathália Luisa,Mathias Melo Eliza,Costa Picossi Carolina Raíssa,Pinto Ernani,Santiago Gomez Ricardo,Martins Teixeira Mauro,Nori de Macedo Adriana,André Baptista Canuto Gisele,Soriani Frederico Marianetti
Abstract
IntroductionPeople with hazardous alcohol use are more susceptible to viral, bacterial, and fungal infections due to the effect of alcohol on immune system cell function. Metabolized ethanol reduces NAD+ to NADH, affecting critical metabolic pathways. Here, our aim was to investigate whether alcohol is metabolized by bone marrow cells and if it impacts the metabolic pathways of leukocyte progenitor cells. This is said to lead to a qualitative and quantitative alteration of key metabolites which may be related to the immune response.MethodsWe addressed this aim by using C57BL/6 mice under chronic ethanol administration and evaluating the metabolomic profile of bone marrow total cells by gas chromatography–coupled mass spectrometry (GC–MS).ResultsWe identified 19 metabolites. Our data demonstrated that chronic ethanol administration alters the metabolomic profile in the bone marrow, resulting in a statistically diminished abundance of five metabolites in ethanol-treated animals: uracil, succinate, proline, nicotinamide, and tyrosine.DiscussionOur results demonstrate for the first time in the literature the effects of alcohol consumption on the metabolome content of hematopoietic tissue and open a wide range of further studies to investigate mechanisms by which alcohol compromises the cellular function of the immune system.
Funder
Conselho Nacional de Desenvolvimento Científico e Tecnológico
Fundação de Amparo à Pesquisa do Estado de Minas Gerais
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior
Publisher
Frontiers Media SA
Subject
Immunology,Immunology and Allergy
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