Author:
Cantoni Diego,Mayora-Neto Martin,Nadesalingam Angalee,Wells David A.,Carnell George W.,Ohlendorf Luis,Ferrari Matteo,Palmer Phil,Chan Andrew C.Y.,Smith Peter,Bentley Emma M.,Einhauser Sebastian,Wagner Ralf,Page Mark,Raddi Gianmarco,Baxendale Helen,Castillo-Olivares Javier,Heeney Jonathan,Temperton Nigel
Abstract
The rise of SARS-CoV-2 variants has made the pursuit to define correlates of protection more troublesome, despite the availability of the World Health Organisation (WHO) International Standard for anti-SARS-CoV-2 Immunoglobulin sera, a key reagent used to standardise laboratory findings into an international unitage. Using pseudotyped virus, we examine the capacity of convalescent sera, from a well-defined cohort of healthcare workers (HCW) and Patients infected during the first wave from a national critical care centre in the UK to neutralise B.1.1.298, variants of interest (VOI) B.1.617.1 (Kappa), and four VOCs, B.1.1.7 (Alpha), B.1.351 (Beta), P.1 (Gamma) and B.1.617.2 (Delta), including the B.1.617.2 K417N, informally known as Delta Plus. We utilised the WHO International Standard for anti-SARS-CoV-2 Immunoglobulin to report neutralisation antibody levels in International Units per mL. Our data demonstrate a significant reduction in the ability of first wave convalescent sera to neutralise the VOCs. Patients and HCWs with more severe COVID-19 were found to have higher antibody titres and to neutralise the VOCs more effectively than individuals with milder symptoms. Using an estimated threshold for 50% protection, 54 IU/mL, we found most asymptomatic and mild cases did not produce titres above this threshold.
Funder
National Institute for Health Research
Subject
Immunology,Immunology and Allergy
Cited by
12 articles.
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