Author:
Li Xiaohan,Chen Zhang,Ye Weicong,Yu Jizhang,Zhang Xi,Li Yuan,Niu Yuqing,Ran Shuan,Wang Song,Luo Zilong,Zhao Jiulu,Hao Yanglin,Zong Junjie,Xia Chengkun,Xia Jiahong,Wu Jie
Abstract
Organ transplantation is the gold standard therapy for end-stage organ failure. However, the shortage of available grafts and long-term graft dysfunction remain the primary barriers to organ transplantation. Exploring approaches to solve these issues is urgent, and CRISPR/Cas9-based transcriptome editing provides one potential solution. Furthermore, combining CRISPR/Cas9-based gene editing with an ex vivo organ perfusion system would enable pre-implantation transcriptome editing of grafts. How to determine effective intervention targets becomes a new problem. Fortunately, the advent of high-throughput CRISPR screening has dramatically accelerated the effective targets. This review summarizes the current advancements, utilization, and workflow of CRISPR screening in various immune and non-immune cells. It also discusses the ongoing applications of CRISPR/Cas-based gene editing in transplantation and the prospective applications of CRISPR screening in solid organ transplantation.
Subject
Immunology,Immunology and Allergy